Stocks closed broadly lower on Wall Street Tuesday as tensions between the U.S. and China flared ahead of negotiations aimed at resolving the costly trade war between the world’s two biggest economies.

The sell-off, which accelerated in the last hour of regular trading, knocked more than 300 points off the Dow Jones Industrial Average. Technology companies, banks and health care stocks bore the brunt of the selling, which stretched the market’s losses further into a fourth week.

The market slide began after the U.S. blacklisted a group of Chinese companies, claiming that their technology plays a role in the repression of China’s Muslim minority groups. The State Department also imposed restrictions on visas for Chinese officials.

The moves cast more doubt on whether Washington and Beijing will find a resolution to their long-running and economically damaging trade conflict. Envoys from the U.S. and China are scheduled to meet in Washington on Thursday for another round of trade talks.

“The rhetoric on both sides, whether it’s the U.S. putting certain Chinese technology companies on a blacklist, or China vowing to retaliate with a ‘stay tuned,’ it just keeps upping the temperature in the room and creating greater uncertainty for businesses, for consumers and for investors,” said Sameer Samana, senior global market strategist at Wells Fargo Investment Institute.

The S&P 500 index lost 45.73 points, or 1.6%, to 2,893.06. The Dow slid 313.98 points, or 1.2%, to 26,164.04. The Nasdaq, which is heavily weighted with technology companies, dropped 132.52 points, or 1.7%, to 7,823.78.

Smaller company stocks were also big decliners, sending the Russell 2000 index down 25.19 points, or 1.7%, to 1,472.60.

The yield on the 10-year Treasury fell to 1.53% from 1.55% late Monday, a signal that investors favored lower-risk investments amid the trade war turmoil.

Utilities and real estate companies, both safe-play sectors, held up better than the rest of the market, though they also ended the day in the red.

The latest escalation in U.S.-China tensions adds yet another worry for investors already anxious over a bevy of political and economic concerns. Last week, the S&P 500 posted its first back-to-back losses of 1% this year as surprisingly weak numbers in surveys of manufacturing and service industries showed the U.S.-China trade war is threatening U.S. economic growth.

Technology stocks were among the biggest losers as chipmakers absorbed the impact of the latest U.S. restrictions on sales to Chinese tech companies that develop facial recognition and other artificial intelligence technology. Ambarella tumbled 9.5% and Nvidia fell 3.9%.

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